do those needles in the haystack actually cause disease?

We seek to leverage high-throughput sequencing data to identify potential variants associated with neurological disorders including autism, epilepsy, and intellectual disability. As the cost of sequencing goes down, our ability to sequence more genomes rises at an exponential rate resulting in thousands of variants of unknown significance. Left with the burden of understanding which variants truly cause disease, we use zebrafish as a tool to assess their impact on gene function, neurodevelopment, and behavior, with the goal to generate high-ish throughput test of function for many variants in parallel.